For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat HTR3A. No significant cross-reactivity or interference between Rat HTR3A and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HTR3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282737 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 3A (HTR3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR3A. The ELISA analytical biochemical technique of the MBS282737 kit is based on HTR3A antibody-HTR3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: For the quantitative detection of Rat 5-hydroxytryptamine receptor 3A (HTR3A) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR3A in Rat serum, plasma. An antibody specific for HTR3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

55,428 Da

5-hydroxytryptamine receptor 3A

5-hydroxytryptamine receptor 3A

5-hydroxytryptamine receptor 3A

5ht3; Htr3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R??[Similar Products]

receptor for 5-hydroxytryptamine (serotonin); functions as a ligand-gated ion channel [RGD, Feb 2006]

5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: axon; cell junction; cell soma; cleavage furrow; cytoplasm; integral component of plasma membrane; plasma membrane; postsynaptic membrane; serotonin-activated cation-selective channel complex

Molecular Function: serotonin binding; serotonin-gated cation-selective channel activity

Biological Process: cation transmembrane transport; cellular response to growth factor stimulus; positive regulation of ion transmembrane transporter activity; response to cocaine; response to ethanol; signal transduction; synaptic transmission

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