Product Name
alpha 1 Antitrypsin (SERPINA1), Polyclonal Antibody
Popular Item
Full Product Name
alpha 1 Antitrypsin antibody
Product Synonym Names
Polyclonal alpha 1 Antitrypsin; Anti-alpha 1 Antitrypsin; A1AT; alpha 1; alpha-1 Antitrypsin; A1-AT
Product Gene Name
anti-SERPINA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01009
Species Reactivity
Baboon, Horse, Mink, Monkey
Form/Format
Purified antibody fraction from rabbit serum supplied in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3.
Concentration
1.0 ml per vial (lot specific)
Biological Significance
Alpha-1-antitrypsin (alpha-1-AT) exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
Immunogen
Alpha 1 antitrypsin antibody was raised in rabbit using purified human serum Alpha-1-AT as the immunogen.
Preparation and Storage
Store at 4 degree C.
Other Notes
Small volumes of anti-SERPINA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SERPINA1 antibody
Rabbit polyclonal alpha 1 Antitrypsin antibody
Product Categories/Family for anti-SERPINA1 antibody
Proteases, Inhibitors, & Enzymes
Applications Tested/Suitable for anti-SERPINA1 antibody
Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes for anti-SERPINA1 antibody
IHC-F: 1:100-1:200
IHC-P: 1:100-1:200
Immunohistochemistry (IHC) of anti-SERPINA1 antibody
Formalin-fixed, paraffin-embedded human tonsil stained with a-1-Antitrypsin (AAT) antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.
NCBI/Uniprot data below describe general gene information for SERPINA1. It may not necessarily be applicable to this product.
NCBI Accession #
AAB59495.1
[Other Products]
UniProt Primary Accession #
P01009
[Other Products]
UniProt Secondary Accession #
Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8[Other Products]
UniProt Related Accession #
P01009[Other Products]
Molecular Weight
34,755 Da[Similar Products]
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol
SERPINA1??[Similar Products]
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
??[Similar Products]
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Synonym Protein Names
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
Protein Family
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1??[Similar Products]
UniProt Synonym Gene Names
AAT; PI; SPAAT??[Similar Products]
UniProt Entry Name
A1AT_HUMAN
NCBI Summary for SERPINA1
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SERPINA1
SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and *****s, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q32.1
Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; glycoprotein binding
Biological Process: platelet activation; response to chromate; platelet degranulation; response to triglyceride; response to cytokine stimulus; response to lead ion; response to hypoxia; acute-phase response; response to methanol; response to lipopolysaccharide; blood coagulation; response to estradiol stimulus
Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive
Research Articles on SERPINA1
1. alpha-1-antitrypsin as substrate of HTRA1 synthetic
Precautions
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Disclaimer
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