Anti -Transcription Factor AP-2 beta (Transcription Factor AP 2 beta, Transcription factor AP2 beta, TFAP2B, Activating Enhancer Binding Protein 2 beta, AP2 beta, AP2beta, AP2-beta, AP2B, AP-2B, AP2-B, MGC21381, OTTHUMP00000039925)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 6; NC_000006.11 (50786439..50815326). Location: 6p12

Monoclonal

IgG

9G453

Mouse

Recognizes human AP2-beta.

Affinity Purified
Purified by affinity chromatography.

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-TFAP2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AP2-beta is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes during early development. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes. AP2-beta appears to be required for normal face and limb development and for proper termnal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. It can form homodimers or heterodimers with other AP-2 family members. Defects in AP2-beta are the cause of Char syndrome (CHAR) which is characterised by patent ductus anteriosus (PDA), facial dysmorphism and hand anomalies.

Antibodies; Abs to Transcription Factors

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in Western Blot and ELISA.

50,474 Da[Similar Products]

transcription factor AP-2 beta (activating enhancer binding protein 2 beta)

transcription factor AP-2-beta; AP2-beta; OTTHUMP00000016595; activating enhancer binding protein 2 beta; activating enhancer-binding protein 2-beta

Transcription factor AP-2-beta

AP2B_HUMAN

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

AP-2 beta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Belongs to the AP-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; caspase inhibitor activity; transcription coactivator activity; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; skin development; hindlimb morphogenesis; positive regulation of transcription, DNA-dependent; response to lithium ion; regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; glucose homeostasis; calcium ion homeostasis; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of cell differentiation; positive regulation of cell proliferation; sodium ion homeostasis; phosphate ion homeostasis; negative regulation of neuron apoptosis; kidney development; potassium ion homeostasis; response to drug; glucose metabolic process; renal water homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; negative regulation of apoptosis

Disease: Char Syndrome

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