MGC20087; MGC35290; PDK1; PRO0461; PkB kinase like gene 1|PkB-like 1|protein kinase

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat PDPK1. No significant cross-reactivity or interference between Rat PDPK1 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of PDPK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285765 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-phosphoinositide-dependent protein kinase 1 (PDPK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDPK1. The ELISA analytical biochemical technique of the MBS285765 kit is based on PDPK1 antibody-PDPK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDPK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDPK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PDPK1 in samples. An antibody specific for PDPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDPK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

63,593 Da

3-phosphoinositide dependent protein kinase-1

3-phosphoinositide-dependent protein kinase 1

3-phosphoinositide-dependent protein kinase 1

Pdk1; PkB kinase??[Similar Products]

may catalyze the phosphorylation and activation of protein kinase B [RGD, Feb 2006]

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: AGC group; EC 2.7.11.1; Kinase, protein; Nuclear receptor co-regulator; PKB family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q12

Cellular Component: cell projection; cytoplasm; cytoplasmic vesicle; cytosol; focal adhesion; nucleus; perikaryon; plasma membrane; postsynaptic density

Molecular Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding; phospholipase activator activity; phospholipase binding; protein kinase binding; protein serine/threonine kinase activity

Biological Process: activation of protein kinase B activity; calcium-mediated signaling; cell migration; cellular response to brain-derived neurotrophic factor stimulus; cellular response to epidermal growth factor stimulus; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; focal adhesion assembly; hyperosmotic response; negative regulation of cardiac muscle cell apoptotic process; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of toll-like receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; platelet activation; positive regulation of phospholipase activity; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid phosphorylation; regulation of endothelial cell migration; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mast cell degranulation; regulation of transcription, DNA-templated; transcription, DNA-dependent; type B pancreatic cell development

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