Anti -Mre11A (DNA Replication, Repair and Recombination Factor)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (94150466..94227040, complement). Location: 11q21

Polyclonal

IgG

Rabbit

Recognizes human Mre11A. Species Crossreactivity: rodent.

Affinity Purified
Purified by affinity chromatography.

Supplied as a liquid in PBS pH 7.2, sodium azide, before the addition of glycerol to 40%.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-MRE11A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity. HMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair.

Antibodies; Abs to DNA Replication, Repair

ELISA (EL/EIA)

Suitable for use in ELISA.
Dilution: ELISA (Serum Titer): 1:250,000
Western Blot: Preliminary results: HeLa cell line: band at 70 and 75kD, OVCAR-3 cell line: band at 70 and 75kD.

59,724 Da[Similar Products]

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

double-strand break repair protein MRE11A; AT-like disease; MRE11 homolog 1; MRE11 homolog A; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein

MRE11A protein

Q05D78_HUMAN

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: Cell cycle regulation; DNA-binding; DNA repair, damage; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; cytosol; chromatin; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: sister chromatid cohesion; positive regulation of kinase activity; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; DNA duplex unwinding; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; base-excision repair; intra-S DNA damage checkpoint; nucleotide-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; meiotic recombination; synapsis; positive regulation of interferon type I production; innate immune response; response to DNA damage stimulus; telomere maintenance

Disease: Ataxia-telangiectasia-like Disorder 1

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.