homeobox protein SIX1; BOS3; DFNA23; TIP39

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 85% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 2M urea

1.0 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of SIX1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SIX1, also known as homeobox protein SIX1, is a member of the SIX gene family. It encodes protein that is characterized by a divergent DNA-binding homeodomain and an upstream SIX domain, which may be involved both in determining DNA-binding specificity and in mediating protein-protein interactions. It has been shown to play roles in vertebrate and insect development or has been implicated in maintenance of the differentiated state of tissues. Recombinant human SIX1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Epigenomics

SDS-PAGE

34.7 kDa (308aa)

SIX homeobox 1

homeobox protein SIX1

Homeobox protein SIX1

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq, Jul 2008]

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (). Regulates the expression of CCNA1.

Ruf RG. et al. (2004) Proc Natl Acad Sci u S A. 101 (21):8090-8095.; Oliver G. et al. (1995) Development. 121 (3):693-705.

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