RET/Proto-oncogene tyrosine-protein kinase receptor Ret/Cadherin family member 12/Proto-oncogene c-Ret/Hirschsprung disease 1/multiple endocrine neoplasia and medullary thyroid carcinoma 1/ret proto-oncogene/RET transforming sequence/CDHR16/MEN2A/MEN2B/CDHF12/CDHR16/PTC/RET51

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RET elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761820 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proto-oncogene tyrosine-protein kinase receptor Ret, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RET. The ELISA analytical biochemical technique of the MBS761820 kit is based on RET antibody-RET antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RET antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RET. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RET antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RET antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RET amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RET can be calculated.

119,847 Da

ret proto-oncogene

proto-oncogene tyrosine-protein kinase receptor Ret

Proto-oncogene tyrosine-protein kinase receptor Ret

CDHF12; CDHR16; PTC; RET51??[Similar Products]

RET_HUMAN

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Oncoprotein; Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: axon; cell soma; cytoplasm; dendrite; early endosome; endosome membrane; integral to plasma membrane; intracellular membrane-bound organelle; lipid raft; plasma membrane; receptor complex

Molecular Function: ATP binding; calcium ion binding; protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: caspase activation; embryonic epithelial tube formation; enteric nervous system development; homophilic cell adhesion; MAPKKK cascade; membrane protein proteolysis; neural crest cell migration; neuron adhesion; neuron maturation; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion mediated by integrin; positive regulation of cell migration; positive regulation of cell size; positive regulation of neuron maturation; positive regulation of transcription, DNA-dependent; posterior midgut development; protein amino acid phosphorylation; regulation of axonogenesis; regulation of cell adhesion; response to drug; response to pain; retina development in camera-type eye; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; ureteric bud development

Disease: Hirschsprung Disease, Susceptibility To, 1; Multiple Endocrine Neoplasia, Type Iia; Multiple Endocrine Neoplasia, Type Iib; Thyroid Carcinoma, Familial Medullary; Thyroid Carcinoma, Papillary

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