For Research Use Only. Not for use in diagnostic procedures.

Sf9 insect cells using baculovirus

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Small volumes of AURA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Recombinant full-length human AURORA A was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
Scientific Background: AURORA A belongs to a multigenic family of mitotic serine/threonine kinases which are involved in the control of chromosome segregation. AURORA A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability (1). AURORA A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of AURORA A has been found to be correlated with the grade of various human solid tumours. Ectopic AURORA A overexpression in any culture cell line leads to polyploidy and centrosome amplification (2).

Enzymes; Active Kinases

Kinase Assay, Western Blot (WB)

~72kDa

aurora kinase A

aurora kinase A

Aurora kinase A

AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1??[Similar Products]

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595.

Protein type: AUR family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: centrosome; condensed nuclear chromosome, pericentric region; cytosol; microtubule cytoskeleton; midbody; nucleoplasm; nucleus; perinuclear region of cytoplasm; spindle; spindle microtubule; spindle midzone; spindle pole centrosome

Molecular Function: histone serine kinase activity; protein binding; protein heterodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G2/M transition of mitotic cell cycle; mitotic cell cycle; mitotic spindle organization and biogenesis; negative regulation of protein binding; positive regulation of mitosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of centrosome cycle; regulation of cytokinesis; regulation of protein stability; response to wounding; spindle organization and biogenesis

Disease: Colorectal Cancer

1. Dutertre, S. et al: On the role of aurora-A in centrosome function. Oncogene. 2002 Sep 9;21 (40):6175-83. 2. Katayama, H. et al: The Aurora kinases: role in cell transformation and tumorigenesis. Cancer Metastasis Rev. 2003 Dec;22 (4):451-64.

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