Full Product Name
Ret Antibody
Product Synonym Names
C-ret; kinase Ret; Proto-oncogene ret precursor
Product Gene Name
anti-Ret antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07949
Species Reactivity
Human, Mouse, Rat
Specificity
Ret Antibody detects endogenous levels of total Ret protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Ret.
Immunogen Range
1041-1090
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-Ret antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Ret antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-Ret antibody
WB: 1:500~1:1000
ELISA: 1:10000
Western Blot (WB) of anti-Ret antibody
Western blot analysis of lysates from K562 cells, using Ret Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for Ret. It may not necessarily be applicable to this product.
NCBI Accession #
NP_065681.1
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NCBI GenBank Nucleotide #
NM_020630.5
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UniProt Primary Accession #
P07949
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UniProt Secondary Accession #
Q15250; Q9BTB0; Q9H4A2; A8K6Z2[Other Products]
UniProt Related Accession #
P07949[Other Products]
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform c
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
RET??[Similar Products]
NCBI Official Synonym Symbols
PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
??[Similar Products]
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Synonym Protein Names
Cadherin family member 12; Proto-oncogene c-Ret
UniProt Gene Name
RET??[Similar Products]
UniProt Synonym Gene Names
CDHF12; CDHR16; PTC; RET51??[Similar Products]
NCBI Summary for Ret
This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis. [provided by RefSeq, Sep 2017]
UniProt Comments for Ret
Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration.
Research Articles on Ret
1. DNA mutational analysis of RET germline mutations associated with medullary thyroid carcinoma in a Druze family.
Precautions
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Disclaimer
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