Full Product Name
RET siRNA (Rat)
Product Synonym Names
Proto-oncogene tyrosine-protein kinase receptor Ret
Product Gene Name
RET sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for G3V9H8
Specificity
RET siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RET gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RET sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RET sirna
siRNA to inhibit RET expression using RNA interference
Applications Tested/Suitable for RET sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RET. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036775.2
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NCBI GenBank Nucleotide #
NM_012643.2
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UniProt Primary Accession #
G3V9H8
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UniProt Secondary Accession #
Q9EPA1; Q9EPC3[Other Products]
UniProt Related Accession #
G3V9H8[Other Products]
Molecular Weight
119,791 Da
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform a
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
Ret??[Similar Products]
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Gene Name
Ret??[Similar Products]
UniProt Entry Name
RET_RAT
NCBI Summary for RET
may play a role in excretory system and enteric nervous system development; human homolog is associated with Hirschsprung disease and Multiple Endocrine Neoplasia type 2 [RGD, Feb 2006]
UniProt Comments for RET
Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.
Protein type: Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Ret family
Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; intracellular; receptor complex; lipid raft
Molecular Function: transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding
Biological Process: regulation of cell adhesion; caspase activation; response to drug; nervous system development; positive regulation of neuron maturation; peptidyl-tyrosine phosphorylation; positive regulation of cell size; membrane protein proteolysis; positive regulation of transcription, DNA-dependent; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; response to pain; regulation of axonogenesis; neuron maturation; enteric nervous system development; neuron differentiation; retina development in camera-type eye; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; homophilic cell adhesion; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration
Research Articles on RET
1. STAT3 phosphorylation by neurturin was found to be mediated specifically by GFRalpha2c and RET9 isoforms.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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