Enamelin; ENAM

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This ENAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1097-1126 amino acids from the C-terminal region of human ENAM.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ENAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC/FACS)

WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50

ENAM Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).

ENAM antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ENAM Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

128785

enamelin

enamelin

Enamelin

ENAM_HUMAN

Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C.[provided by RefSeq, Oct 2009]

enamelin: Involved in the mineralization and structural organization of enamel. Involved in the extension of enamel during the secretory stage of dental enamel formation. Defects in ENAM are the cause of amelogenesis imperfecta type 1B (AI1B). AI1B is an autosomal dominant defect of enamel formation. Clinical manifestations may be variable. Some cases present with generalized enamel hypoplasia resulting in small, smooth, yellow and spaced teeth (smooth hypoplastic AI). Others show horizontal rows of pits, grooves or a hypoplastic area in the enamel (local hypoplastic AI). Defects in ENAM are the cause of amelogenesis imperfecta type 1C (AI1C); also known as amelogenesis imperfecta hypoplastic with or without openbite malocclusion. AI1C is an autosomal recessive defect of dental enamel formation. Teeth show hypoplastic and unmineralized enamel, and a yellow-brown discoloration. Enamel defects can be associated with facial and oral features including vertical dysgnathia and anterior openbite malocclusion.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: proteinaceous extracellular matrix

Biological Process: biomineral formation

Disease: Amelogenesis Imperfecta, Type Ic; Amelogenesis Imperfecta, Type Ib

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