For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C.

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Small volumes of PNPLA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9137638 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PNPLA3. The ELISA analytical biochemical technique of the MBS9137638 kit is based on PNPLA3 antibody-PNPLA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PNPLA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PNPLA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) in the sample, this Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

52,485 Da

patatin like phospholipase domain containing 3

1-acylglycerol-3-phosphate O-acyltransferase PNPLA3

1-acylglycerol-3-phosphate O-acyltransferase PNPLA3

ADPN1 PublicationManual assertion based on opinion iniRef.10; iPLA2-epsilon1 PublicationManual assertion based on opinion iniRef.7??[Similar Products]

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]

Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed:22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed:15364929, PubMed:20034933, PubMed:22560221).

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