Aldehyde dehydrogenase; Aldehyde dehydrogenase 10; ALDH10; ALDH3A2; FALDH; Fatty aldehyde dehydrogenase; Microsomal aldehyde dehydrogenase; SLS; P51648; Fatty aldehyde dehydrogenase; aldehyde dehydrogenase 3 family member A2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ALDH3A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit IgG polyclonal antibody for Fatty aldehyde dehydrogenase (ALDH3A2) detection.
Background: Fatty aldehyde dehydrogenase (or Long-chain-aldehyde dehydrogenase) is an aldehyde dehydrogenase enzyme that in human is encoded in the ALDH3A2 gene on chromosome 17. ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes into fatty acids. It is known to act on a variety of both saturated and unsaturated aliphatic aldehydes between 6 to 24 carbons in length, as well as dihydrophytal, a 20-carbon branched chain aldehyde. It requires NAD+ as a co-factor. The encoded enzyme is responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. ALD3H2 is expressed in the human liver and has been found to localize the microsome fraction inside the cell.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Rat
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western blot analysis of ALDH3A2 expression in rat liver extract (lane 1) and 22RV1 whole cell lysates (lane 2). ALDH3A2 at 55KD was detected using rabbit anti- ALDH3A2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

ALDH3A2 was detected in paraffin-embedded sections of human liver cancer tissues using rabbit anti- ALDH3A2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

57,669 Da

aldehyde dehydrogenase 3 family member A2

fatty aldehyde dehydrogenase

Fatty aldehyde dehydrogenase

ALDH10; FALDH??[Similar Products]

AL3A2_HUMAN

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - glycerolipid; Oxidoreductase; Amino Acid Metabolism - histidine; Mitochondrial; Amino Acid Metabolism - tryptophan; Other Amino Acids Metabolism - beta-alanine; EC 1.2.1.3; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Membrane protein, integral; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; peroxisomal membrane

Molecular Function: 3-chloroallyl aldehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; long-chain-alcohol oxidase activity

Biological Process: aldehyde metabolic process; central nervous system development; epidermis development; fatty acid alpha-oxidation; peripheral nervous system development; phytol metabolic process; sphingolipid biosynthetic process

Disease: Sjogren-larsson Syndrome

1. Kelson TL, Secor McVoy JR, Rizzo WB (1997). "Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization". Biochim. Biophys. Acta 1335 (1-2): 99-110.
2. Rogers GR, Markova NG, De Laurenzi V, Rizzo WB, Compton JG (1997). "Genomic organization and expression of the human fatty aldehyde dehydrogenase gene (FALDH)". Genomics 39 (2): 127-35.

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