Product Name
FAM83H, siRNA
Full Product Name
FAM83H siRNA (Mouse)
Product Synonym Names
Protein FAM83H
Product Gene Name
FAM83H sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q148V8
Specificity
FAM83H siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FAM83H gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FAM83H sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FAM83H sirna
siRNA to inhibit FAM83H expression using RNA interference
Applications Tested/Suitable for FAM83H sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FAM83H. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161725.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001168253.1
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UniProt Primary Accession #
Q148V8
[Other Products]
UniProt Secondary Accession #
Q8BZF6; Q8CI79; Q8R5C2; Q8R5D0[Other Products]
UniProt Related Accession #
Q148V8[Other Products]
Molecular Weight
131,115 Da
NCBI Official Full Name
protein FAM83H
NCBI Official Synonym Full Names
family with sequence similarity 83, member H
NCBI Official Symbol
Fam83h??[Similar Products]
NCBI Official Synonym Symbols
AA409316
??[Similar Products]
NCBI Protein Information
protein FAM83H
UniProt Protein Name
Protein FAM83H
UniProt Gene Name
Fam83h??[Similar Products]
UniProt Entry Name
FA83H_MOUSE
UniProt Comments for FAM83H
FAM83H: May play a major role in the structural organization and calcification of developing enamel. Defects in FAM83H are the cause of amelogenesis imperfecta type 3 (AI3). AI3 is an autosomal dominant hypomineralized form of amelogenesis imperfecta, a defect of enamel formation. AI3 is characterized by enamel of normal thickness, but soft and with cheesy consistency. Enamel is lost from tooth soon after eruption. Belongs to the FAM83 family.
Protein type: Unknown function
Biological Process: biomineral formation
Research Articles on FAM83H
1. Findings demonstrate that over-expression of FAM83H in mice does not produce a phenotype in dentine or enamel.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
