For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ALDH3A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against ALDH3A2. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid.

Polyclonal; Membrane Protein; Drugs and Drug Metabolism;

Western Blot (WB)

Rabbit Anti-ALDH3A2 antibody
Formalin Fixed Paraffin Embedded Tissue: Human Adult Adrenal
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Host: Rabbit
Target Name: ALDH3A2
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: ALDH3A2
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

WB Suggested Anti-ALDH3A2 Antibody
Positive Control: Lane1: 30ug human primary hepatocytes
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:5000
Submitted by: Takeshi Saito, USC Keck School of Medicine

WB Suggested Anti-ALDH3A2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: NCI-H226 cell lysate

58kDa

aldehyde dehydrogenase 3 family member A2

fatty aldehyde dehydrogenase

Fatty aldehyde dehydrogenase

ALDH10; FALDH??[Similar Products]

AL3A2_HUMAN

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - histidine; Membrane protein, integral; Oxidoreductase; EC 1.2.1.3; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine; Lipid Metabolism - fatty acid; Amino Acid Metabolism - arginine and proline; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrial inner membrane; integral to membrane; peroxisome

Molecular Function: long-chain-alcohol oxidase activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; long-chain-aldehyde dehydrogenase activity

Biological Process: phytol metabolic process; epidermis development; central nervous system development; aldehyde metabolic process; sesquiterpenoid metabolic process; peripheral nervous system development

Disease: Sjogren-larsson Syndrome

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