For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-Ptase.

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.
Stable for at least 1 year at -20 degree C.

Small volumes of anti-NCC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr53, Thr58, and Ser71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).

Western Blot (WB)

WB: 1:1000

Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda- Ptase, 1200 units for 30 min).

~160 kDa

solute carrier family 12, member 3

solute carrier family 12 member 3

Solute carrier family 12 member 3

Ncc; Tsc??[Similar Products]

Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (PubMed:26099046).

Feng X, Zhang Y, Shao N, Wang Y, Zhuang Z, Wu P, Lee MJ, Liu Y, Wang X, Zhuang J, Delpire E, Gu D, Cai H. (2015) Aldosterone modulates thiazide-sensitive sodium chloride cotransporter abundance via DUSP6-mediated ERK1/2 signaling pathway. Am J Physiol Renal Physiol. 308(10):F1119-27
Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA. (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem. 289(19):13347-61

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