Product Name
ACAD9, siRNA
Full Product Name
ACAD9 siRNA (Human)
Product Synonym Names
Acyl-CoA dehydrogenase family member 9 mitochondrial; ACAD-9
Product Gene Name
ACAD9 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9H845
Specificity
ACAD9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACAD9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACAD9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACAD9 sirna
siRNA to inhibit ACAD9 expression using RNA interference
Applications Tested/Suitable for ACAD9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACAD9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_054768.2
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NCBI GenBank Nucleotide #
NM_014049.4
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UniProt Primary Accession #
Q9H845
[Other Products]
UniProt Secondary Accession #
Q8WXX3; D3DNB8[Other Products]
UniProt Related Accession #
Q9H845[Other Products]
Molecular Weight
68,760 Da
NCBI Official Full Name
acyl-CoA dehydrogenase family member 9, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase family, member 9
NCBI Official Symbol
ACAD9??[Similar Products]
NCBI Official Synonym Symbols
NPD002
??[Similar Products]
NCBI Protein Information
acyl-CoA dehydrogenase family member 9, mitochondrial
UniProt Protein Name
Acyl-CoA dehydrogenase family member 9, mitochondrial
Protein Family
Acyl-CoA dehydrogenase family
UniProt Gene Name
ACAD9??[Similar Products]
UniProt Synonym Gene Names
ACAD-9??[Similar Products]
UniProt Entry Name
ACAD9_HUMAN
NCBI Summary for ACAD9
This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
UniProt Comments for ACAD9
ACAD9: Has a dehydrogenase activity on palmitoyl-CoA (C16:0) and stearoyl-CoA (C18:0). It is three times more active on palmitoyl-CoA then on stearoyl-CoA. Has little activity on octanoyl-CoA (C8:0), butyryl-CoA (C4:0) or isovaleryl-CoA (5:0). Belongs to the acyl-CoA dehydrogenase family.
Protein type: Oxidoreductase; Mitochondrial; EC 1.3.99.-
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: mitochondrion; dendrite; nucleus
Molecular Function: acyl-CoA dehydrogenase activity; protein binding; FAD binding
Biological Process: mitochondrial respiratory chain complex I assembly
Disease: Acyl-coa Dehydrogenase Family, Member 9, Deficiency Of
Research Articles on ACAD9
1. Our results underscore the importance of the ACAD9 protein in complex I assembly and suggest that the enzymatic activity is a rudiment of the duplication event.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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