Product Name
MBNL1, Polyclonal Antibody
Popular Item
Full Product Name
MBNL1 Polyclonal Antibody
Product Gene Name
anti-MBNL1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NR56
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human MBNL1
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-MBNL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MBNL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF)
Application Notes for anti-MBNL1 antibody
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Western Blot (WB) of anti-MBNL1 antibody
Western blot analysis of extracts of various cell lines, using MBNL1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry (IHC) of anti-MBNL1 antibody
Immunohistochemistry of paraffin-embedded rat heart using MBNL1 antibody at dilution of 1:100 (40x lens).
NCBI/Uniprot data below describe general gene information for MBNL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_066368.2
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NCBI GenBank Nucleotide #
NM_021038.3
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UniProt Primary Accession #
Q9NR56
[Other Products]
UniProt Secondary Accession #
O43311; O43797; Q86UV8; Q86UV9; Q96P92; Q96RE3; E9PBW7[Other Products]
UniProt Related Accession #
Q9NR56[Other Products]
NCBI Official Full Name
muscleblind-like protein 1 isoform a
NCBI Official Synonym Full Names
muscleblind-like splicing regulator 1
NCBI Official Symbol
MBNL1??[Similar Products]
NCBI Official Synonym Symbols
EXP; MBNL; EXP35; EXP40; EXP42
??[Similar Products]
NCBI Protein Information
muscleblind-like protein 1
UniProt Protein Name
Muscleblind-like protein 1
UniProt Synonym Protein Names
Triplet-expansion RNA-binding protein
Protein Family
Muscleblind-like protein
UniProt Gene Name
MBNL1??[Similar Products]
UniProt Synonym Gene Names
EXP; KIAA0428; MBNL??[Similar Products]
UniProt Entry Name
MBNL1_HUMAN
UniProt Comments for MBNL1
MBNL1: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y- 3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Plays a role in the pathogenesis of dystrophia myotonica type 1 (DM1). A muscular disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness and cardiac arrhythmias. In muscle cells from DM1 patients, MBNL1 is sequestered by DMPK RNAs containing CUG triplet repeat expansions. MBNL1 binding is proportional to repeat length consistent with the direct correlation between the length of repeat expansion and disease severity. Belongs to the muscleblind family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 3q25
Cellular Component: nucleoplasm; centrosome; stress granule; cytoplasm; nucleus
Molecular Function: protein binding; RNA binding; metal ion binding; double-stranded RNA binding
Biological Process: myoblast differentiation; alternative nuclear mRNA splicing, via spliceosome; mRNA splice site selection; nervous system development; skeletal muscle development; regulation of RNA splicing; in utero embryonic development; RNA splicing; regulation of alternative nuclear mRNA splicing, via spliceosome; embryonic limb morphogenesis
Research Articles on MBNL1
1. Results show that nuclear localization is a major determinant of MBNL1 function. It promotes the nuclear retention of repeat-containing transcripts, which results in repression of aberrant protein expression from the expanded repeats.
Precautions
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Disclaimer
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