Product Name
ALDH3A2, siRNA
Full Product Name
ALDH3A2 siRNA (Rat)
Product Synonym Names
ALDH4; Fatty aldehyde dehydrogenase; Aldehyde dehydrogenase 4; Aldehyde dehydrogenase family 3 member A2; Microsomal aldehyde dehydrogenase; msALDH
Product Gene Name
ALDH3A2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P30839
Specificity
ALDH3A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ALDH3A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALDH3A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALDH3A2 sirna
siRNA to inhibit ALDH3A2 expression using RNA interference
Applications Tested/Suitable for ALDH3A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALDH3A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_113919.2
[Other Products]
NCBI GenBank Nucleotide #
NM_031731.2
[Other Products]
UniProt Primary Accession #
P30839
[Other Products]
UniProt Related Accession #
P30839[Other Products]
Molecular Weight
54,082 Da
NCBI Official Full Name
fatty aldehyde dehydrogenase
NCBI Official Synonym Full Names
aldehyde dehydrogenase 3 family, member A2
NCBI Official Symbol
Aldh3a2??[Similar Products]
NCBI Official Synonym Symbols
Aldh4; FALDH
??[Similar Products]
NCBI Protein Information
fatty aldehyde dehydrogenase
UniProt Protein Name
Fatty aldehyde dehydrogenase
UniProt Synonym Protein Names
Aldehyde dehydrogenase 4; Aldehyde dehydrogenase family 3 member A2; Microsomal aldehyde dehydrogenase; msALDH
Protein Family
Fatty aldehyde dehydrogenase
UniProt Gene Name
Aldh3a2??[Similar Products]
UniProt Synonym Gene Names
Aldh4; msALDH??[Similar Products]
UniProt Entry Name
AL3A2_RAT
UniProt Comments for ALDH3A2
ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Oxidoreductase; Endoplasmic reticulum; Mitochondrial; Amino Acid Metabolism - arginine and proline; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Other Amino Acids Metabolism - beta-alanine; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Lipid Metabolism - fatty acid; EC 1.2.1.3; Carbohydrate Metabolism - butanoate
Cellular Component: intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; peroxisome; cytosol; nucleus
Molecular Function: long-chain-alcohol oxidase activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; long-chain-aldehyde dehydrogenase activity
Biological Process: phytol metabolic process; response to reactive oxygen species; epidermis development; central nervous system development; formaldehyde metabolic process; aldehyde metabolic process; sesquiterpenoid metabolic process; peripheral nervous system development
Research Articles on ALDH3A2
1. FALDH has roles in insulin action and is deregulated in states associated with altered insulin signaling
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
