Mouse Aminopeptidase N (ANPEP) ELISA kit; APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M; aminopeptidase N; membrane alanine aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; alanyl (membrane) aminopeptidase

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse ANPEP. No significant cross-reactivity or interference between mouse ANPEP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ANPEP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS911187 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alanyl (membrane) aminopeptidase (ANPEP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANPEP. The ELISA analytical biochemical technique of the MBS911187 kit is based on ANPEP antibody-ANPEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANPEP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANPEP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

109,651 Da

alanyl (membrane) aminopeptidase

aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase

Aminopeptidase N

Lap-1; Lap1; AP-N; mAPN; AP-M??[Similar Products]

AMPN_MOUSE

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Membrane protein, integral; Protease; Other Amino Acids Metabolism - glutathione; EC 3.4.11.2; Receptor, misc.

Cellular Component: extracellular space; membrane; lysosomal membrane; integral to membrane; ER-Golgi intermediate compartment; vesicle membrane; external side of plasma membrane

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; aminopeptidase activity; peptide binding

Biological Process: multicellular organismal development; aromatic compound metabolic process; angiogenesis; cell differentiation; proteolysis

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