Product Name
ABCD4, siRNA
Full Product Name
ABCD4 siRNA (Mouse)
Product Synonym Names
PXMP1L; ATP-binding cassette sub-family D member 4; PMP70-related protein; P70R; Peroxisomal membrane protein 1-like; PXMP1-L; Peroxisomal membrane protein 69; PMP69
Product Gene Name
ABCD4 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O89016
Specificity
ABCD4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABCD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ABCD4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ABCD4 sirna
siRNA to inhibit ABCD4 expression using RNA interference
Applications Tested/Suitable for ABCD4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ABCD4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033018.2
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NCBI GenBank Nucleotide #
NM_008992.2
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UniProt Primary Accession #
O89016
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UniProt Secondary Accession #
E9QKU3[Other Products]
UniProt Related Accession #
O89016[Other Products]
Molecular Weight
68,583 Da
NCBI Official Full Name
ATP-binding cassette sub-family D member 4
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family D (ALD), member 4
NCBI Official Symbol
Abcd4??[Similar Products]
NCBI Official Synonym Symbols
P69r; P70R; Pxmp1l
??[Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family D member 4
UniProt Protein Name
ATP-binding cassette sub-family D member 4
UniProt Synonym Protein Names
PMP70-related protein; P70R; Peroxisomal membrane protein 1-like; PXMP1-L; Peroxisomal membrane protein 69; PMP69
Protein Family
ATP-binding cassette sub-family
UniProt Gene Name
Abcd4??[Similar Products]
UniProt Synonym Gene Names
Pxmp1l; P70R; PXMP1-L; PMP69??[Similar Products]
UniProt Entry Name
ABCD4_MOUSE
NCBI Summary for ABCD4
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that the human protein may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for ABCD4
ABCD4: is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq, Jul 2008]
Protein type: Membrane protein, multi-pass; Transporter, ABC family; Transporter; Membrane protein, integral
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane; peroxisome
Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATPase activity; nucleotide binding; ATP binding
Biological Process: cobalamin metabolic process; transport; transmembrane transport
Research Articles on ABCD4
1. P70R resides in the ER but not the peroxisomal membranes, and the hydrophobic property of NH(2)-terminal region determines the subcellular localization of ABC subfamily D proteins.
Precautions
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Disclaimer
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