Product Name
SLC12A3, Polyclonal Antibody
Full Product Name
SLC12A3 Antibody
Product Synonym Names
Solute carrier family 12 member 3; Na-Cl cotransporter; NCC; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter; SLC12A3; NCC; TSC
Product Gene Name
anti-SLC12A3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P55017
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant Human Solute carrier family 12 member 3 protein (791-952AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-SLC12A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SLC12A3 antibody
Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (PubMed:21613606, PubMed:22009145). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (By similarity).
Product Categories/Family for anti-SLC12A3 antibody
Cardiovascular; Metabolism; Signal transduction
Applications Tested/Suitable for anti-SLC12A3 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes for anti-SLC12A3 antibody
IF: 1:50-1:200
Immunofluorescence (IF) of anti-SLC12A3 antibody
Immunofluorescence staining of Hela cells with MBS7109016 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
NCBI/Uniprot data below describe general gene information for SLC12A3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000330.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000339.2
[Other Products]
UniProt Primary Accession #
P55017
[Other Products]
UniProt Secondary Accession #
A8MSJ2; C9JNN9[Other Products]
UniProt Related Accession #
P55017[Other Products]
Molecular Weight
113,808 Da
NCBI Official Full Name
solute carrier family 12 member 3 isoform 1
NCBI Official Synonym Full Names
solute carrier family 12 member 3
NCBI Official Symbol
SLC12A3??[Similar Products]
NCBI Official Synonym Symbols
NCC; TSC; NCCT
??[Similar Products]
NCBI Protein Information
solute carrier family 12 member 3
UniProt Protein Name
Solute carrier family 12 member 3
UniProt Synonym Protein Names
Na-Cl cotransporter; NCC; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
Protein Family
Solute carrier family
UniProt Gene Name
SLC12A3??[Similar Products]
UniProt Synonym Gene Names
NCC; TSC; NCC??[Similar Products]
NCBI Summary for SLC12A3
This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SLC12A3
Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (PubMed:21613606, PubMed:22009145). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 ().
Research Articles on SLC12A3
1. A were found in transcript NM_000339.2 in SLC12A3 gene were found in a patient with Gitelman syndrome. The first mutation was also found in patient's motherand the second in father. Only one of the two mutations iden-tified in our patient c.35 36insA was found in his sister.">2 novel heterozygous mutations: c.35_36insA and c.1095+5G>A were found in transcript NM_000339.2 in SLC12A3 gene were found in a patient with Gitelman syndrome. The first mutation was also found in patient's motherand the second in father. Only one of the two mutations iden-tified in our patient c.35 36insA was found in his sister.
Precautions
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