ATP-binding cassette sub-family G member 5; ABCG5; ABCG5_HUMAN; ATP binding cassette sub family G (WHITE) member 5 (sterolin 1); ATP binding cassette sub family G member 5; ATP-binding cassette sub-family G member 5; Sterolin 1; Sterolin-1; STSL antibody; ATP-binding cassette, sub-family G (WHITE), member 5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Immunogen Affinity Purified

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ABCG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 5(ABCG5) detection. Tested with WB in Human.

Background: ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.

Western Blot (WB)

Western Blot Concentration: 0.1-0.5ug/ml

Anti- ABCG5 Picoband antibody, MBS177958, Western blotting
All lanes: Anti ABCG5 (MBS177958) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted bind size: 73KD
Observed bind size: 73KD

28,480 Da

ATP binding cassette subfamily G member 5

ATP-binding cassette sub-family G member 5

ATP-binding cassette sub-family G member 5

ABCG5_HUMAN

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

ABCG5: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Defects in ABCG5 are a cause of sitosterolemia (STSL); also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Protein type: Transporter; Membrane protein, integral; Transporter, ABC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: apical plasma membrane; ATP-binding cassette (ABC) transporter complex; plasma membrane; receptor complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; cholesterol transporter activity; protein binding; protein heterodimerization activity

Biological Process: cholesterol absorption; cholesterol efflux; cholesterol homeostasis; excretion; metabolic process; negative regulation of cholesterol absorption; response to drug; response to ionizing radiation; response to nutrient; sterol transport; transmembrane transport

Disease: Sitosterolemia

1. Lee, M.-H., Lu, K., Hazard, S., Yu, H., Shulenin, S., Hidaka, H., Kojima, H., Allikmets, R., Sakuma, N., Pegoraro, R., Srivastava, A. K., Salen, G., Dean, M., Patel, S. B. Identification of a gene, ABCG5, important in the regulation of dietary cholesterol absorption. Nature Genet. 27: 79-83, 2001. 2. Lu, K., Lee, M. H., Yu, H., Zhou, Y., Sandell, S. A., Salen, G., Patel, S. B. Molecular cloning, genomic organization, genetic variations, and characterization of murine sterolin genes Abcg5and Abcg8. J. Lipid Res. 43: 565-578, 2002. 3. Small, D. M. Role of ABC transporters in secretion of cholesterol from liver into bile. (Commentary)Proc. Nat. Acad. Sci. 100: 4-6, 2003.

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