ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G(WHITE), member 5; ABCG5 antibody; ABCG5_HUMAN antibody; ATP binding cassette sub family G(WHITE) member 5(sterolin 1) antibody; ATP binding cassette sub family G member 5 antibody; ATP-binding cassette sub-family G member 5 antibody; Sterolin 1 antibody; Sterolin-1 antibody; STSL antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ABCG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 5(ABCG5) detection. Tested with WB in Human.
Background: ABCG5(Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1,?is a?protein that in humans is encoded by the ABCG5?gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters.?This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The?ABCG5?gene contains 13 exons and spans about 28 kb.The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small(2003)?reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of?ABCG5/ABCG8.The?ABCG5?and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.

Western Blot (WB)

Figure 1. Western blot analysis of ABCG5 using anti- ABCG5 antibody (MBS176226).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate
Lane 2: A549 Whole Cell Lysate
Lane 3: HT1080 Whole Cell Lysate
Lane 4: U87 Whole Cell Lysate
Lane 5: SKOV Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ABCG5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.

Figure 2. Flow Cytometry analysis of A431 cells using anti-ABCG5 antibody (MBS176226).
Overlay histogram showing A431 cells stained with MBS176226 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG5 Antibody (MBS176226,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight?488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

28,480 Da

ATP-binding cassette, sub-family G (WHITE), member 5

ATP-binding cassette sub-family G member 5; sterolin 1; sterolin-1; ATP-binding cassette, subfamily G, member 5

ATP-binding cassette sub-family G member 5

ABCG5_HUMAN

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

ABCG5: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Defects in ABCG5 are a cause of sitosterolemia (STSL); also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Protein type: Membrane protein, integral; Transporter, ABC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: apical plasma membrane; plasma membrane; ATP-binding cassette (ABC) transporter complex; receptor complex

Molecular Function: protein binding; protein heterodimerization activity; cholesterol transporter activity; ATPase activity; ATP binding

Biological Process: response to drug; negative regulation of cholesterol absorption; cholesterol homeostasis; cholesterol absorption; cholesterol efflux; response to ionizing radiation; excretion; transmembrane transport; response to nutrient; sterol transport

Disease: Sitosterolemia

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.