Purified Rabbit Polyclonal Antibody (Pab); Proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycan; Proteoglycan 4 C-terminal part; PRG4; MSF; SZP

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit

This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.

Purified through a protein A column, followed by peptide affinity purification.

Supplied in PBS with 0.09% (W/V) sodium azide.

Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-PRG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.

New Antibodies; Primary Antibodies; Signal Transduction

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

IF: 1:25
WB: 1:500

Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 151 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

151077

proteoglycan 4

proteoglycan 4

Proteoglycan 4

MSF; SZP??[Similar Products]

The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.MiscellaneousDifferent forms varying in molecular weight have been observed. Such forms are possibly due to different levels of glycosylation and protein cleavage ().

Turner K.J.,et al. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Jones A.R.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Flannery C.R.,et al.Biochem. Biophys. Res. Commun. 254:535-541(1999).

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