Product Name
Thiopurine S-methyltransferase (Tpmt), Recombinant Protein
Full Product Name
Recombinant Mouse Thiopurine S-methyltransferase (Tpmt)
Product Gene Name
Tpmt recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-240aa, Full length protein
Sequence
MSLDMKEHPD AEVQKNQVLT LEDWKEKWVT RHISFHQEQG HQLLKKHLDT FLKGQSGLRV FFPLCGKAIE MKWFADRGHT VVGVEISEIG IREFFAEQNL SYTEEPLAEI AGAKVFKSSS GSISLYCCSI FDLPRANIGK FDRIWDRGAL VAINPGDHDR YADIILSLLR KEFQYLVAVL SYDPTKHAGP PFYVPSAELK RLFGTKCSMQ CLEEVDALEE RHKAWGLDYL FEKLYLLTEK
3D Structure
ModBase 3D Structure for O55060
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Tpmt recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Tpmt recombinant protein
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.
NCBI/Uniprot data below describe general gene information for Tpmt. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058065.2
[Other Products]
NCBI GenBank Nucleotide #
NM_016785.2
[Other Products]
UniProt Primary Accession #
O55060
[Other Products]
UniProt Secondary Accession #
Q9JIL7; Q9QUG7[Other Products]
UniProt Related Accession #
O55060[Other Products]
Molecular Weight
27,586 Da
NCBI Official Full Name
thiopurine S-methyltransferase
NCBI Official Synonym Full Names
thiopurine methyltransferase
NCBI Official Symbol
Tpmt??[Similar Products]
NCBI Official Synonym Symbols
AW106912
??[Similar Products]
NCBI Protein Information
thiopurine S-methyltransferase
UniProt Protein Name
Thiopurine S-methyltransferase
UniProt Synonym Protein Names
Thiopurine methyltransferase
UniProt Gene Name
Tpmt??[Similar Products]
UniProt Comments for Tpmt
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Research Articles on Tpmt
1. With the significant mitigation of TPMT wild type-expressing cells to cisplatin cytotoxicity, findings demonstrate a drug-gene interaction between increased TPMT activity and decreased susceptibility to cisplatin-induced toxicity of inner ear cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
