Product Name
AMHR2, siRNA
Full Product Name
AMHR2 siRNA (Rat)
Product Synonym Names
Anti-Muellerian hormone type-2 receptor; Anti-Muellerian hormone type II receptor; AMH type II receptor; C14; MIS type II receptor; MISRII; MRII
Product Gene Name
AMHR2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62893
Specificity
AMHR2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AMHR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AMHR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AMHR2 sirna
siRNA to inhibit AMHR2 expression using RNA interference
Applications Tested/Suitable for AMHR2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AMHR2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_112260.1
[Other Products]
NCBI GenBank Nucleotide #
NM_030998.1
[Other Products]
UniProt Primary Accession #
Q62893
[Other Products]
UniProt Secondary Accession #
Q63045; Q9R0A7[Other Products]
UniProt Related Accession #
Q62893[Other Products]
Molecular Weight
59,749 Da
NCBI Official Full Name
anti-Muellerian hormone type-2 receptor
NCBI Official Synonym Full Names
anti-Mullerian hormone receptor, type II
NCBI Official Symbol
Amhr2??[Similar Products]
NCBI Protein Information
anti-Muellerian hormone type-2 receptor
UniProt Protein Name
Anti-Muellerian hormone type-2 receptor
UniProt Synonym Protein Names
Anti-Muellerian hormone type II receptor; AMH type II receptor; C14; MIS type II receptor; MISRII; MRII
Protein Family
Anti-Muellerian hormone type-2 receptor
UniProt Gene Name
Amhr2??[Similar Products]
UniProt Synonym Gene Names
AMH type II receptor; MISRII; MRII??[Similar Products]
UniProt Entry Name
AMHR2_RAT
NCBI Summary for AMHR2
receptor that binds Mullerian inhibiting substance; involved in testis and ovary function [RGD, Feb 2006]
UniProt Comments for AMHR2
MISR2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Protein type: Protein kinase, TKL; Membrane protein, integral; EC 2.7.11.30; Kinase, protein; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily
Cellular Component: integral to plasma membrane
Molecular Function: transforming growth factor beta receptor activity, type II; hormone binding; metal ion binding; receptor activity; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: G-protein coupled receptor protein signaling pathway; transforming growth factor beta receptor signaling pathway; signal transduction; protein amino acid phosphorylation; sex differentiation
Research Articles on AMHR2
1. physiological function(s) of type 2 anti-Mullerian hormone receptor
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
