Full Product Name
ALAD siRNA (Rat)
Product Synonym Names
Delta-aminolevulinic acid dehydratase; ALADH; Porphobilinogen synthase
Product Gene Name
ALAD sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P06214
Specificity
ALAD siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ALAD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALAD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALAD sirna
siRNA to inhibit ALAD expression using RNA interference
Applications Tested/Suitable for ALAD sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALAD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037031.1
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NCBI GenBank Nucleotide #
NM_012899.2
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UniProt Primary Accession #
P06214
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UniProt Related Accession #
P06214[Other Products]
Molecular Weight
36,032 Da
NCBI Official Full Name
delta-aminolevulinic acid dehydratase
NCBI Official Synonym Full Names
aminolevulinate dehydratase
NCBI Official Symbol
Alad??[Similar Products]
NCBI Official Synonym Symbols
ALADR; aminolevulinatedelta-dehydratase
??[Similar Products]
NCBI Protein Information
delta-aminolevulinic acid dehydratase
UniProt Protein Name
Delta-aminolevulinic acid dehydratase
UniProt Synonym Protein Names
Porphobilinogen synthase
UniProt Gene Name
Alad??[Similar Products]
UniProt Synonym Gene Names
ALADH??[Similar Products]
UniProt Entry Name
HEM2_RAT
NCBI Summary for ALAD
catalyzes the conversion of 5-aminolevulinate to porphobilinogen in heme biosynthesis [RGD, Feb 2006]
UniProt Comments for ALAD
ALAD: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Defects in ALAD are the cause of acute hepatic porphyria (AHEPP). A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. Belongs to the ALADH family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lyase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 4.2.1.24
Cellular Component: extracellular space; cytosol; nucleus
Molecular Function: identical protein binding; porphobilinogen synthase activity; zinc ion binding; lead ion binding
Biological Process: response to metal ion; response to toxin; response to arsenic; response to glucocorticoid stimulus; response to hormone stimulus; response to lipopolysaccharide; response to organic cyclic substance; response to organic substance; response to mercury ion; response to vitamin B1; response to vitamin; response to vitamin E; response to selenium ion; response to methylmercury; response to iron ion; response to nutrient; protein homooligomerization; response to aluminum ion; response to nutrient levels; response to drug; response to inorganic substance; response to herbicide; response to amino acid stimulus; response to cadmium ion; response to ethanol; response to zinc ion; response to lead ion; response to hypoxia; response to cobalt ion; response to activity; response to ionizing radiation; response to oxidative stress; protoporphyrinogen IX biosynthetic process; heme biosynthetic process
Research Articles on ALAD
1. the inhibition of catalase, delta aminolevulinic acid dehydratase (eth-ALA-D) and isoforms of lactate dehydrogenase (LDH) accompanied by hyperglycemia were prevented by DPDS in all tissues examined.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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