Full Product Name
ALAD siRNA (Mouse)
Product Synonym Names
LV; Delta-aminolevulinic acid dehydratase; ALADH; Porphobilinogen synthase
Product Gene Name
ALAD sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10518
Specificity
ALAD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALAD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALAD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALAD sirna
siRNA to inhibit ALAD expression using RNA interference
Applications Tested/Suitable for ALAD sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALAD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001263375.1
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NCBI GenBank Nucleotide #
NM_001276446.1
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UniProt Primary Accession #
P10518
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UniProt Secondary Accession #
Q3THV6[Other Products]
UniProt Related Accession #
P10518[Other Products]
Molecular Weight
36,024 Da
NCBI Official Full Name
delta-aminolevulinic acid dehydratase
NCBI Official Synonym Full Names
aminolevulinate, delta-, dehydratase
NCBI Official Symbol
Alad??[Similar Products]
NCBI Official Synonym Symbols
Lv; ALADH
??[Similar Products]
NCBI Protein Information
delta-aminolevulinic acid dehydratase
UniProt Protein Name
Delta-aminolevulinic acid dehydratase
UniProt Synonym Protein Names
Porphobilinogen synthase
UniProt Gene Name
Alad??[Similar Products]
UniProt Synonym Gene Names
Lv; ALADH??[Similar Products]
UniProt Entry Name
HEM2_MOUSE
UniProt Comments for ALAD
ALAD: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Defects in ALAD are the cause of acute hepatic porphyria (AHEPP). A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. Belongs to the ALADH family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lyase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 4.2.1.24
Cellular Component: extracellular space; nucleus; cytosol
Molecular Function: identical protein binding; porphobilinogen synthase activity; zinc ion binding; metal ion binding; lyase activity; lead ion binding; catalytic activity
Biological Process: response to drug; response to organic substance; response to metal ion; response to ethanol; response to cadmium ion; response to zinc ion; metabolic process; response to arsenic; tetrapyrrole biosynthetic process; porphyrin biosynthetic process; response to nutrient; protein homooligomerization; heme biosynthetic process
Research Articles on ALAD
1. Similar to its role at the beta-globin promoter, KLF1 induces factor recruitment and chromatin changes at the Alad1b promoter in a temporally-specific manner
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
