Human Cocaine-and amphetamine-regulated transcript protein (Cartpt) ELISA kit; CART; cocaine and amphetamine regulated transcript; cocaine- and amphetamine-regulated transcript; Cocaine-and amphetamine-regulated transcript protein (Cartpt)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human Cartpt. No significant cross-reactivity or interference between human Cartpt and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Cartpt elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS904739 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cocaine-and amphetamine-regulated transcript protein (Cartpt) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cartpt. The ELISA analytical biochemical technique of the MBS904739 kit is based on Cartpt antibody-Cartpt antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cartpt antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cartpt. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Cartpt has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cartpt present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cartpt is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cartpt bound in the initial step. The color development is stopped and the intensity of the color is measured.

12,829 Da

CART prepropeptide

cocaine- and amphetamine-regulated transcript protein; cocaine and amphetamine regulated transcript

Cocaine- and amphetamine-regulated transcript protein

CART??[Similar Products]

CART_HUMAN

This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.[provided by RefSeq, Nov 2009]

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Hormone; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: extracellular space; secretory granule

Molecular Function: protein binding

Biological Process: negative regulation of osteoclast differentiation; activation of MAPKK activity; ***** feeding behavior; cellular response to starvation; signal transduction; negative regulation of appetite; G-protein coupled receptor protein signaling pathway; synaptic transmission; cell-cell signaling; neuropeptide signaling pathway; positive regulation of epinephrine secretion; cell glucose homeostasis; circadian regulation of gene expression; negative regulation of bone resorption; regulation of insulin secretion; positive regulation of blood pressure; positive regulation of transmission of nerve impulse

Disease: Obesity

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