Full Product Name
ApoB Antibody
Product Synonym Names
FLDB; LDLCQ4
Product Gene Name
anti-ApoB antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P04114
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Immunogen
Purified recombinant fragment of human ApoB expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Other Notes
Small volumes of anti-ApoB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ApoB antibody
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Product Categories/Family for anti-ApoB antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-ApoB antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-ApoB antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Western Blot (WB) of anti-ApoB antibody
Figure 1: Western blot analysis using ApoB mAb against recombinant protein.
Immunofluorescence (IF) of anti-ApoB antibody
Figure 2: Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Testing Data of anti-ApoB antibody
NCBI/Uniprot data below describe general gene information for ApoB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000375.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000384.2
[Other Products]
UniProt Primary Accession #
P04114
[Other Products]
UniProt Secondary Accession #
O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8[Other Products]
UniProt Related Accession #
P04114[Other Products]
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
APOB??[Similar Products]
NCBI Official Synonym Symbols
FLDB; LDLCQ4; apoB-48; apoB-100
??[Similar Products]
NCBI Protein Information
apolipoprotein B-100
UniProt Protein Name
Apolipoprotein B-100
Protein Family
Apolipoprotein
UniProt Gene Name
APOB??[Similar Products]
UniProt Synonym Gene Names
Apo B-100; Apo B-48??[Similar Products]
NCBI Summary for ApoB
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
UniProt Comments for ApoB
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Research Articles on ApoB
1. Results show differences in the apoB100 secondary structure content among lipoproteins incubated at pH 7.4 or at pH 5.0 that could resemble those occurring in the early or late endosomes, respectively.
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Disclaimer
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