Product Name
TMEM67, siRNA
Full Product Name
TMEM67 siRNA (Human)
Product Synonym Names
MKS3; Meckelin; Meckel syndrome type 3 protein; Transmembrane protein 67
Product Gene Name
TMEM67 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5HYA8
Specificity
TMEM67 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMEM67 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TMEM67 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TMEM67 sirna
siRNA to inhibit TMEM67 expression using RNA interference
Applications Tested/Suitable for TMEM67 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TMEM67. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001135773.1
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NCBI GenBank Nucleotide #
NM_001142301.1
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UniProt Primary Accession #
Q5HYA8
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UniProt Secondary Accession #
Q3ZCX3; Q7Z5T8; Q8IZ06; B3KRU5; B3KT47; G5E9H2[Other Products]
UniProt Related Accession #
Q5HYA8[Other Products]
Molecular Weight
103,590 Da
NCBI Official Full Name
meckelin isoform 2
NCBI Official Synonym Full Names
transmembrane protein 67
NCBI Official Symbol
TMEM67??[Similar Products]
NCBI Official Synonym Symbols
MKS3; JBTS6; NPHP11; TNEM67; MECKELIN
??[Similar Products]
NCBI Protein Information
meckelin
UniProt Protein Name
Meckelin
UniProt Synonym Protein Names
Meckel syndrome type 3 protein; Transmembrane protein 67
UniProt Gene Name
TMEM67??[Similar Products]
UniProt Synonym Gene Names
MKS3??[Similar Products]
UniProt Entry Name
MKS3_HUMAN
NCBI Summary for TMEM67
The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq, Nov 2008]
UniProt Comments for TMEM67
TMEM67: a protein localizes to the primary cilium and to the plasma membrane. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (SFTPC). Ciliary dysfunction leads to a broad spectrum of disorders, collectively termed ciliopathies. Defects in this protein are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). Overlapping clinical features include retinal degeneration, renal cystic disease, skeletal abnormalities, fibrosis of various organ, and a complex range of anatomical and functional defects of the central and peripheral nervous system. Interacts with DNAJB9, DNAJC10, MKS1 and mutated SFTPC. Interacts with SYNE2 during the early establishment of cell polarity.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8q22.1
Cellular Component: centrosome; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; integral to membrane
Molecular Function: protein binding; unfolded protein binding; filamin binding
Biological Process: ER-associated protein catabolic process; organelle organization and biogenesis; cilium biogenesis
Disease: Coach Syndrome; Meckel Syndrome, Type 3; Nephronophthisis 11; Bardet-biedl Syndrome 1; Joubert Syndrome 6
Research Articles on TMEM67
1. The Meckel syndrome protein meckelin (TMEM67) is a key regulator of cilia function but is not required for tissue planar polarity.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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