Full Product Name
TMC1 siRNA (Mouse)
Product Synonym Names
BTH; DN; Transmembrane channel-like protein 1; Beethoven protein; Deafness protein; Transmembrane cochlear-expressed protein 1
Product Gene Name
TMC1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8R4P5
Specificity
TMC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TMC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TMC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TMC1 sirna
siRNA to inhibit TMC1 expression using RNA interference
Applications Tested/Suitable for TMC1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TMC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_083229.1
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NCBI GenBank Nucleotide #
NM_028953.2
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UniProt Primary Accession #
Q8R4P5
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UniProt Secondary Accession #
Q7TQB2; Q9D435[Other Products]
UniProt Related Accession #
Q8R4P5[Other Products]
Molecular Weight
31,965 Da
NCBI Official Full Name
transmembrane channel-like protein 1
NCBI Official Synonym Full Names
transmembrane channel-like gene family 1
NCBI Official Symbol
Tmc1??[Similar Products]
NCBI Official Synonym Symbols
dn; Bth; CWEA1; Beethoven; 4933416G09Rik
??[Similar Products]
NCBI Protein Information
transmembrane channel-like protein 1
UniProt Protein Name
Transmembrane channel-like protein 1
UniProt Synonym Protein Names
Beethoven protein; Deafness protein; Transmembrane cochlear-expressed protein 1
Protein Family
AN1-type zinc finger protein
UniProt Gene Name
Tmc1??[Similar Products]
UniProt Synonym Gene Names
Bth; dn??[Similar Products]
UniProt Entry Name
TMC1_MOUSE
UniProt Comments for TMC1
TMC1: Required for the normal function of cochlear hair cells. Defects in TMC1 are the cause of deafness autosomal dominant type 36 (DFNA36). DFNA36 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA36 is a bilateral hearing loss, and begins at 5-10 years of age. It progresses to profound deafness within 10-15 years. Defects in TMC1 are the cause of deafness autosomal recessive type 7 (DFNB7); also known as autosomal recessive neurosensory deafness type 11 (DFNB11). This hearing loss is congenital and profound. Belongs to the TMC family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: membrane; integral to membrane; stereocilium bundle tip; external side of plasma membrane
Molecular Function: voltage-gated calcium channel activity
Biological Process: sensory perception of sound; vestibular reflex; transport; auditory receptor cell development; ion transport; detection of mechanical stimulus involved in sensory perception of sound
Disease: Deafness, Autosomal Recessive 7
Research Articles on TMC1
1. During the first postnatal week, we observed a normal mechanotransducer current in hair cells lacking Tmc1 or Tmc2; however, in the absence of both isoforms, we recorded a large MT current that was phase-shifted 180 degrees .
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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