Goat Antibody to Human Apolipoprotein B (Apo B)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (21224301..21266945, complement). Location: 2p24-p23

Polyclonal

Goat

Apolipoprotein B (Apo B) (Monospecific by IEP with neat antibody versus 2X pooled human serum and neat pooled human plasma.)

Defribrinated, delipidized and adsorbed by solid phase chromatography. Product is 0.2um filtered.

Monospecific, Liquid

Total protein: 8.0 g/dL (lot specific)

Short term (up to 7 days) store at 2-8 degree C. Long term, aliquot and store at -20 degree C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freeze/thaw cycles.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-APOB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal Antibodies to Lipoproteins

515,605 Da[Similar Products]

apolipoprotein B (including Ag(x) antigen)

apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48

Apolipoprotein B-100

Apo B-100; Apo B-48??[Similar Products]

APOB_HUMAN

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Subcellular location: Secreted.

Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.34

Post-translational modification: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. Ref.31

Involvement in disease: Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1) [

MIM:107730]. A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Ref.46Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [

MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Ref.40 Ref.42 Ref.44 Ref.49Note=Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Sequence similarities: Contains 1 vitellogenin domain.

RNA editing: Edited at position 2180.The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.

Sequence caution: The sequence AAA51752.1 differs from that shown. Reason: Frameshift at positions 942, 951, 1139, 1165, 1164, 1371 and 1385.

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