For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection ofCFHR3. No significant cross-reactivity or interference betweenCFHR3and analogues was observed.

Store at 4 degree C for 6 months.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CFHR3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7607060 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CFHR3 (Complement factor H-related protein 3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CFHR3. The ELISA analytical biochemical technique of the MBS7607060 kit is based on CFHR3 antibody-CFHR3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFHR3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFHR3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CFHR3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CFHR3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CFHR3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CFHR3 can be calculated.

30,720 Da

complement factor H related 3

complement factor H-related protein 3

Complement factor H-related protein 3

CFHL3; FHR3; FHR-3??[Similar Products]

The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Might be involved in complement regulation.

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