5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Tau-protein kinase PRKAA1; Prkaa1; 2.7.11.1; ACACA kinase; 2.7.11.27; Hydroxymethylglutaryl-CoA reductase

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Prkaa1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2881158 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5'-AMP-activated protein kinase catalytic subunit alpha-1 (Prkaa1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prkaa1. The ELISA analytical biochemical technique of the MBS2881158 kit is based on Prkaa1 antibody-Prkaa1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prkaa1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Prkaa1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

63,929 Da

protein kinase, AMP-activated, alpha 1 catalytic subunit

5'-AMP-activated protein kinase catalytic subunit alpha-1

5'-AMP-activated protein kinase catalytic subunit alpha-1

AMPK subunit alpha-1; ACACA kinase; HMGCR kinase??[Similar Products]

AAPK1_MOUSE

AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protein kinase, CAMK; EC 2.7.11.26; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; AMPK subfamily

Cellular Component: AMP-activated protein kinase complex; apical plasma membrane; cytoplasm; nucleus; protein complex

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; histone serine kinase activity; kinase activity; kinase binding; metal ion binding; nucleotide binding; protein binding; protein C-terminus binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: autophagy; cellular response to glucose starvation; cellular response to nutrient levels; cholesterol biosynthetic process; cholesterol metabolic process; chromatin modification; cold acclimation; fatty acid biosynthetic process; fatty acid homeostasis; fatty acid metabolic process; fatty acid oxidation; glucose homeostasis; glucose metabolic process; lipid biosynthetic process; lipid metabolic process; negative regulation of apoptosis; negative regulation of lipid catabolic process; negative regulation of TOR signaling pathway; phosphorylation; positive regulation of autophagy; positive regulation of cell proliferation; positive regulation of glycolysis; positive regulation of skeletal muscle development; protein amino acid phosphorylation; protein heterooligomerization; regulation of circadian rhythm; regulation of peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; response to activity; response to caffeine; response to gamma radiation; response to hydrogen peroxide; response to UV; rhythmic process; steroid biosynthetic process; steroid metabolic process; sterol biosynthetic process; transcription, DNA-dependent; Wnt receptor signaling pathway

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