Full Product Name
BOLL siRNA (Human)
Product Synonym Names
BOULE; Protein boule-like
Product Gene Name
BOLL sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8N9W6
Specificity
BOLL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BOLL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BOLL sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BOLL sirna
siRNA to inhibit BOLL expression using RNA interference
Applications Tested/Suitable for BOLL sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BOLL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001271287.1
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NCBI GenBank Nucleotide #
NM_001284358.1
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UniProt Primary Accession #
Q8N9W6
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UniProt Secondary Accession #
Q0JW32; Q53T62; Q969U3; B4DZA4[Other Products]
UniProt Related Accession #
Q8N9W6[Other Products]
Molecular Weight
19,256 Da
NCBI Official Full Name
protein boule-like isoform 3
NCBI Official Synonym Full Names
boule-like RNA-binding protein
NCBI Official Symbol
BOLL??[Similar Products]
NCBI Official Synonym Symbols
BOULE
??[Similar Products]
NCBI Protein Information
protein boule-like
UniProt Protein Name
Protein boule-like
Protein Family
Protein boule
UniProt Gene Name
BOLL??[Similar Products]
UniProt Synonym Gene Names
BOULE??[Similar Products]
UniProt Entry Name
BOLL_HUMAN
NCBI Summary for BOLL
This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BOLL
BOLL: Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Belongs to the RRM DAZ family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoplasm
Molecular Function: protein binding; translation activator activity; RNA binding; nucleotide binding
Biological Process: positive regulation of translational initiation; meiosis; multicellular organismal development; spermatogenesis; cell differentiation
Research Articles on BOLL
1. Our study demonstrated an oncogenic role of BOLL in CRC despite tumor-specific promoter hypermethylation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
