Product Name
BOULE / BOLL, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human BOULE / BOLL
Product Synonym Names
Anti-BOULE / BOLL Antibody (C-Terminus) IHC-plus; BOLL; Bol; boule-like (Drosophila); Protein boule-like; BOULE; Human BOULE; BOLL
Product Gene Name
anti-BOULE antibody
[Similar Products]
Product Synonym Gene Name
BOLL[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8N9W6
Species Reactivity
Gibbon, Bovine, Chimpanzee, Dog, Human, Mouse
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Specificity
Human BOLL / BOULE. This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide C-QPEPIKTVWSIHY from the C-terminus of human BOLL / BOULE (NP_932074.1; NP_149019.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Tamarin, Mouse, Dog, Bovine, Opossum (100%); Marmoset (92%).
Immunogen Type
Synthetic peptide
Immunogen
BOULE / BOLL antibody was raised against synthetic peptide C-QPEPIKTVWSIHY from the C-terminus of human BOLL / BOULE (NP_932074.1; NP_149019.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Tamarin, Mouse, Dog, Bovine, Opossum (100%); Marmoset (92%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-BOULE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BOULE antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-BOULE antibody
ELISA (1:32000), IHC-P (3.75 ug/ml)
Immunohistochemistry (IHC) of anti-BOULE antibody
Anti-BOLL / BOULE antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
NCBI/Uniprot data below describe general gene information for BOULE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001271287.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001284358.1
[Other Products]
UniProt Primary Accession #
Q8N9W6
[Other Products]
UniProt Secondary Accession #
Q0JW32; Q53T62; Q969U3; B4DZA4[Other Products]
UniProt Related Accession #
Q8N9W6[Other Products]
Molecular Weight
19,256 Da
NCBI Official Full Name
protein boule-like isoform 3
NCBI Official Synonym Full Names
boule-like RNA-binding protein
NCBI Official Symbol
BOLL??[Similar Products]
NCBI Official Synonym Symbols
BOULE
??[Similar Products]
NCBI Protein Information
protein boule-like; bol, boule-like
UniProt Protein Name
Protein boule-like
UniProt Gene Name
BOLL??[Similar Products]
UniProt Synonym Gene Names
BOULE??[Similar Products]
UniProt Entry Name
BOLL_HUMAN
NCBI Summary for BOULE
This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BOULE
BOLL: Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Belongs to the RRM DAZ family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoplasm
Molecular Function: protein binding; translation activator activity; RNA binding; nucleotide binding
Biological Process: positive regulation of translational initiation; meiosis; multicellular organismal development; spermatogenesis; cell differentiation
Research Articles on BOULE
1. demonstrate that Boll is also transiently expressed during oogenesis in the fetal mouse ovary, but is simultaneously co-expressed within the same germ cells as Dazl
Precautions
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Disclaimer
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