Product Name
BPIFA1, siRNA
Full Product Name
BPIFA1 siRNA (Rat)
Product Synonym Names
PLUNC; BPI fold-containing family A member 1; Palate lung and nasal epithelium clone protein
Product Gene Name
BPIFA1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8K4I4
Specificity
BPIFA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BPIFA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BPIFA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BPIFA1 sirna
siRNA to inhibit BPIFA1 expression using RNA interference
Applications Tested/Suitable for BPIFA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BPIFA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_742028.1
[Other Products]
NCBI GenBank Nucleotide #
NM_172031.1
[Other Products]
UniProt Primary Accession #
Q8K4I4
[Other Products]
UniProt Related Accession #
Q8K4I4[Other Products]
Molecular Weight
27,719 Da
NCBI Official Full Name
BPI fold-containing family A member 1
NCBI Official Synonym Full Names
BPI fold containing family A, member 1
NCBI Official Symbol
Bpifa1??[Similar Products]
NCBI Official Synonym Symbols
Plunc
??[Similar Products]
NCBI Protein Information
BPI fold-containing family A member 1
UniProt Protein Name
BPI fold-containing family A member 1
UniProt Synonym Protein Names
Palate lung and nasal epithelium clone protein
Protein Family
BPI fold-containing family
UniProt Gene Name
Bpifa1??[Similar Products]
UniProt Synonym Gene Names
Plunc??[Similar Products]
UniProt Entry Name
BPIA1_RAT
NCBI Summary for BPIFA1
localized to the microvilli layer of nasal epithelium; may play a role in response to injury [RGD, Feb 2006]
UniProt Comments for BPIFA1
Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
