Full Product Name
Anti - CLC1 (Clcn1, Chloride Channel)
Product Synonym Names
CLC1 (Clcn1, Chloride Channel)
Product Gene Name
anti-CLC1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 7; NC_000007.13 (143013219..143049097). Location: 7q32-qter; 7q35
3D Structure
ModBase 3D Structure for P35523
Host
Host: Rabbit
Source: Rat
Specificity
The 19 AA rat control peptide 94% conserved in mouse and 84% in human CLC-1. No significant sequence homology is detected with other CLCs or other proteins. Because of the low MW (<3kD) of the control peptide, it is not recommended for Western. The control peptide is available to confirm specificity of antibodies
Purity/Purification
Purified over the peptide-Sepharose column
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium azide, 40% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A 19 AA Peptide sequence near the N-terminus of rat CLC-1 (1). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-CLC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CLC1 antibody
Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen\'s disease), cystic fibrosis, Bartters syndrome type III, Dent\'s disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
Product Categories/Family for anti-CLC1 antibody
Abs to Ion Channel
Applications Tested/Suitable for anti-CLC1 antibody
ELISA, Western Blot
Application Notes for anti-CLC1 antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for CLC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000074.2
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NCBI GenBank Nucleotide #
NM_000083.2
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UniProt Primary Accession #
P35523
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UniProt Secondary Accession #
Q2M202; A4D2H5[Other Products]
UniProt Related Accession #
P35523; Q75L28[Other Products]
Molecular Weight
108,626 Da
NCBI Official Full Name
chloride channel protein 1
NCBI Official Synonym Full Names
chloride channel 1, skeletal muscle
NCBI Official Symbol
CLCN1??[Similar Products]
NCBI Official Synonym Symbols
CLC1; MGC138361; MGC142055
??[Similar Products]
NCBI Protein Information
chloride channel protein 1; clC-1; OTTHUMP00000202223; chloride channel protein, skeletal muscle
UniProt Protein Name
Chloride channel protein 1
UniProt Synonym Protein Names
Chloride channel protein, skeletal muscle
UniProt Gene Name
CLCN1??[Similar Products]
UniProt Synonym Gene Names
CLC1??[Similar Products]
UniProt Entry Name
CLCN1_HUMAN
Product References and Citations for anti-CLC1 antibody
??Steinmeyer K et al (1991) Nature 354, 301; Koch MC et al (1992) Science 257, 797; George AL et al (1993) Nature Genet. 3, 305; Steinmeyer K et al (1994) EMBO J 13, 737; Lorenz C et al (1994) Human Mol. Genet. 3, 941; Heine R et al (1994) Hum. Mol. Genet. 3, 1123; George Al et al (1994) Hum. Mol. Genet. 3, 2071; Meyer-Kleine C et al (1995) Am. J. Hum. Genet. 57, 1325; Lehman Horn F et al (1995) Hum. Mol. Genet 4, 1397; Mailander V et al (1996)) Am. J. Hum. Genet. 58, 317
Precautions
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Disclaimer
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