Full Product Name
HAMSTER ANTI MOUSE CD154:FITC
Product Gene Name
anti-CD154 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P27548
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Immunogen
L cells transfected with CD40L
Buffer Solution
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD154 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD154 antibody
Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39kDa cell surface glycoprotein. CD154 is expressed by activated CD4+ve T lymphocytes. Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.
Applications Tested/Suitable for anti-CD154 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD154 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat
NCBI/Uniprot data below describe general gene information for CD154. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035746.2
[Other Products]
NCBI GenBank Nucleotide #
NM_011616.2
[Other Products]
UniProt Primary Accession #
P27548
[Other Products]
UniProt Related Accession #
P27548[Other Products]
Molecular Weight
29,370 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
Cd40lg??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
??[Similar Products]
NCBI Protein Information
CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
UniProt Gene Name
Cd40lg??[Similar Products]
UniProt Synonym Gene Names
Cd40l; Tnfsf5; CD40-L; TRAP??[Similar Products]
UniProt Entry Name
CD40L_MOUSE
UniProt Comments for CD154
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
Protein type: Membrane protein, integral; Cell adhesion
Cellular Component: extracellular space; cell surface; membrane; cell; integral to membrane; extracellular region; plasma membrane; external side of plasma membrane
Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding
Biological Process: B cell proliferation; platelet activation; positive regulation of interleukin-12 production; isotype switching; positive regulation of immune response to tumor cell; signal transduction; positive regulation of interleukin-10 production; positive regulation of interferon-gamma production; B cell differentiation; positive regulation of MHC class II biosynthetic process; immunoglobulin secretion; positive regulation of B cell proliferation; immune response; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis
Research Articles on CD154
1. data show that vaccination with TAA/CD40L DNA can induce potent antitumor effects against TAA-expressing tumors through the generation of better functioning antigen-specific CD8+ T cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
