Product Name
CD154 (CD40LG), Polyclonal Antibody
Full Product Name
Anti-CD154 Antibody
Product Synonym Names
CD40L; TNFSF5; TRAP; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154
Product Gene Name
anti-CD40LG antibody
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Antibody/Peptide Pairs
CD154 peptide (MBS824223) is used for blocking the activity of CD154 antibody (MBS822072)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P29965
Species Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Specificity
Recognizes endogenous levels of CD154 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD154. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CD40LG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD40LG antibody
Rabbit polyclonal antibody to CD154
Applications Tested/Suitable for anti-CD40LG antibody
Western Blot (WB)
Application Notes for anti-CD40LG antibody
WB (1/500 - 1/1000)
Western Blot (WB) of anti-CD40LG antibody
Western blot analysis of CD154 expression in HEK293T (A), mouse spleen (B), rat spleen (C) whole cell lysates.
NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000065.1
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NCBI GenBank Nucleotide #
NM_000074.2
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UniProt Primary Accession #
P29965
[Other Products]
UniProt Secondary Accession #
P27548; Q9Z2V2[Other Products]
UniProt Related Accession #
P29965[Other Products]
Molecular Weight
29,274 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
??[Similar Products]
NCBI Protein Information
CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
Protein Family
CD40 ligand
UniProt Gene Name
CD40LG??[Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]
UniProt Entry Name
CD40L_HUMAN
NCBI Summary for CD40LG
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for CD40LG
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: Xq26
Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; immunoglobulin secretion; B cell differentiation; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis
Disease: Immunodeficiency With Hyper-igm, Type 1
Research Articles on CD40LG
1. In chronic hepatitis C, the hepatocyte infection rate influences OSMRb expression levels, which also show variability among patients.
Precautions
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Disclaimer
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