Full Product Name
Human LAMA1 EZ-Set ELISA Kit (DIY Antibody Pairs)
Product Synonym Names
Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2; Lamb2;
LAMB2_HUMAN;
LAMC1;
LAMC2;
LAMC3; laminin A;
Laminin B;
Laminin B1s chain;
Laminin gamma 1;
Laminin gamma 2;
Laminin gamma 3;
Laminin subunit beta-2;
Laminin-11 subunit beta;
Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta;
Laminin-9 subunit beta;
OCCM; S-LAM beta;
S-laminin subunit beta; Laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2 (laminin S)
Product Gene Name
LAMA1 antibody pair kit
[Similar Products]
Product Synonym Gene Name
LAMA2; LAMB1; LAMB2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for B0YJ33
Specificity
Natural and recombinant human LAMA1
Assay Range
156pg/ml-10000pg/ml
Immunogen
Expression system for standard: from human fibroblasts
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |1335|4334|7066|1728|4566|7324
Standard Deviation: |72.09|286|296.8|110.6|342.5|366.2
CV(%): |5.4|6.6|4.2|6.4|7.5|5
Sample Data
Concentration (pg/ml): |0|156|312|625|1250|2500|5000|10000
OD: |0.095|0.137|0.168|0.247|0.425|0.624|1.284|2.110
Preparation Step 2
2. Reconstitution of human LAMA1 standard
1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10ng of lyophilized human LAMA1 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Human LAMA1 Standard
? Number tubes 1-8. Final Concentrations to be Tube # 1 -10000pg/ml, #2 -5000pg/ml, #3 - 2500pg/ml, #4 - 1250pg/ml, #5 - 625pg/ml, #6 - 312pg/ml, #7 - 156pg/ml, #8 - 0.0 (Blank)
? For standard #1, add 1000ul of undiluted standard stock solution to tube #1
? Add 300 ul of sample diluent to tubes # 2-7
? To generate standard #2, add 300 ul of standard #1 from tube #1 to tube #2 for a final volume of 600 ul. Mix thoroughly
? To generate standard #3, add 300 ul of standard #2 from tube #2 to tube #3 for a final volume of 600 ul. Mix thoroughly
? Continue the serial dilution for tube #4-7
? Tube #8 is a blank standard to be used with every experiment.
Preparation Step 2
2. Reconstitution of human LAMA1 standard
1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10ng of lyophilized human LAMA1 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Human LAMA1 Standard
? Number tubes 1-8. Final Concentrations to be Tube # 1 -10000pg/ml, #2 -5000pg/ml, #3 - 2500pg/ml, #4 - 1250pg/ml, #5 - 625pg/ml, #6 - 312pg/ml, #7 - 156pg/ml, #8 - 0.0 (Blank)
? For standard #1, add 1000ul of undiluted standard stock solution to tube #1
? Add 300 ul of sample diluent to tubes # 2-7
? To generate standard #2, add 300 ul of standard #1 from tube #1 to tube #2 for a final volume of 600 ul. Mix thoroughly
? To generate standard #3, add 300 ul of standard #2 from tube #2 to tube #3 for a final volume of 600 ul. Mix thoroughly
? Continue the serial dilution for tube #4-7
? Tube #8 is a blank standard to be used with every experiment.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months
Avoid multiple freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LAMA1 antibody pair kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LAMA1 antibody pair kit
Description: For the development of sandwich ELISA kit to measure human LAMA1 in cell culture supernates, cell lysates and serum.
Background: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse
biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
NCBI/Uniprot data below describe general gene information for LAMA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000218.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000227.4
[Other Products]
UniProt Primary Accession #
B0YJ33
[Other Products]
UniProt Secondary Accession #
Q13679; Q13680; Q6VU67; Q6VU68; Q6VU69; Q76E14; Q96TG0; B0YJ33[Other Products]
UniProt Related Accession #
Q16787[Other Products]
Molecular Weight
190,491 Da
NCBI Official Full Name
laminin subunit alpha-3 isoform 2
NCBI Official Synonym Full Names
laminin subunit alpha 3
NCBI Official Symbol
LAMA3??[Similar Products]
NCBI Official Synonym Symbols
E170; LOCS; BM600; LAMNA
??[Similar Products]
NCBI Protein Information
laminin subunit alpha-3
UniProt Protein Name
Laminin subunit alpha-3
UniProt Synonym Protein Names
Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
UniProt Gene Name
LAMA3??[Similar Products]
UniProt Synonym Gene Names
LAMNA; E170??[Similar Products]
NCBI Summary for LAMA1
The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Comments for LAMA1
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Research Articles on LAMA1
1. Seventeen percent of the patients were compound heterozygous or homozygous for mutations in the gene LAMA3, 59% carried mutations in both alleles of LAMB3, and 12% were homozygous for mutations in LAMC2. In nine patients with severe generalized JEB, detection of two mutations in one of the genes LAMA3, LAMB3, or LAMC2 was not possible, so the molecular basis of disease could not be clarified completely
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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