Full Product Name
Mouse LAMA1 EZ-Set ELISA Kit (DIY Antibody Pairs)
Product Synonym Names
Laminin subunit alpha-3; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; Lama3; Laminin, alpha 1
Product Gene Name
LAMA1 antibody pair kit
[Similar Products]
Product Synonym Gene Name
LAMA2; LAMB1; LAMB2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for E9PUR4
Specificity
Natural and recombinant mouse LAMA1
Assay Range
156pg/ml-10000pg/ml
Immunogen
Expression system for standard: from murine sarcoma basement membrane
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |1266|3866|6831|1576|4364|7224
Standard Deviation: |58.24|204.9|457.7|91.41|270.6|534.6
CV(%): |4.6|5.3|6.7|5.8|6.2|7.4
Sample Data
Concentration (pg/ml): |0|156|312|625|1250|2500|5000|10000
OD: |0.083|0.135|0.182|0.243|0.430|0.773|1.277|1.958
Preparation Step 2
2. Reconstitution of mouse LAMA1 standard
1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10ng of lyophilized mouse LAMA1 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Mouse LAMA1 Standard
? Number tubes 1-8. Final Concentrations to be Tube # 1 -10000pg/ml, #2 -5000pg/ml, #3 - 2500pg/ml, #4 - 1250pg/ml, #5 - 625pg/ml, #6 - 312pg/ml, #7 - 156pg/ml, #8 - 0.0 (Blank)
? For standard #1, add 1000ul of undiluted standard stock solution to tube #1
? Add 300 ul of sample diluent to tubes # 2-7
? To generate standard #2, add 300 ul of standard #1 from tube #1 to tube #2 for a final volume of 600 ul. Mix thoroughly
? To generate standard #3, add 300 ul of standard #2 from tube #2 to tube #3 for a final volume of 600 ul. Mix thoroughly
? Continue the serial dilution for tube #4-7
? Tube #8 is a blank standard to be used with every experiment.
Preparation Step 2
2. Reconstitution of mouse LAMA1 standard
1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10ng of lyophilized mouse LAMA1 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Mouse LAMA1 Standard
? Number tubes 1-8. Final Concentrations to be Tube # 1 -10000pg/ml, #2 -5000pg/ml, #3 - 2500pg/ml, #4 - 1250pg/ml, #5 - 625pg/ml, #6 - 312pg/ml, #7 - 156pg/ml, #8 - 0.0 (Blank)
? For standard #1, add 1000ul of undiluted standard stock solution to tube #1
? Add 300 ul of sample diluent to tubes # 2-7
? To generate standard #2, add 300 ul of standard #1 from tube #1 to tube #2 for a final volume of 600 ul. Mix thoroughly
? To generate standard #3, add 300 ul of standard #2 from tube #2 to tube #3 for a final volume of 600 ul. Mix thoroughly
? Continue the serial dilution for tube #4-7
? Tube #8 is a blank standard to be used with every experiment.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months
Avoid multiple freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LAMA1 antibody pair kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LAMA1 antibody pair kit
Description: For the development of sandwich ELISA kit to measure mouse LAMA1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Background: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse
biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.
NCBI/Uniprot data below describe general gene information for LAMA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001334390.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001347461.1
[Other Products]
UniProt Primary Accession #
E9PUR4
[Other Products]
UniProt Secondary Accession #
O08751; Q61788; Q61966; Q9JHQ7; E9PUR4[Other Products]
UniProt Related Accession #
Q61789[Other Products]
Molecular Weight
190,610 Da
NCBI Official Full Name
laminin subunit alpha-3 isoform 3A
NCBI Official Synonym Full Names
laminin, alpha 3
NCBI Official Symbol
Lama3??[Similar Products]
NCBI Official Synonym Symbols
[a]3B; Lama3B
??[Similar Products]
NCBI Protein Information
laminin subunit alpha-3
UniProt Protein Name
Laminin subunit alpha-3
UniProt Synonym Protein Names
Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
UniProt Gene Name
Lama3??[Similar Products]
UniProt Comments for LAMA1
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Research Articles on LAMA1
1. the gradual disappearance of alpha3 chain-containing laminins along the dermal-epidermal junction and formation of subepidermal blisters like in congenital junctional epidermis bullosa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
