Full Product Name
LAMA3 Antibody
Product Synonym Names
Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Nicein subunit alpha; LAMA3; LAMNA
Product Gene Name
anti-LAMA3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16787
Species Reactivity
Human, Mouse, Rat
Specificity
LAMA3 Antibody detects endogenous levels of total LAMA3 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human LAMA3.
Immunogen Range
2571-2620
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-LAMA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LAMA3 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-LAMA3 antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Immunofluorescence (IF) of anti-LAMA3 antibody
Immunofluorescence analysis of HepG2 cells, using LAMA3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (IHC) of anti-LAMA3 antibody
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using LAMA3 Antibody. The picture on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for LAMA3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000218.3
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NCBI GenBank Nucleotide #
NM_000227.4
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UniProt Primary Accession #
Q16787
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UniProt Secondary Accession #
Q13679; Q13680; Q6VU67; Q6VU68; Q6VU69; Q76E14; Q96TG0; B0YJ33[Other Products]
UniProt Related Accession #
Q16787[Other Products]
NCBI Official Full Name
laminin subunit alpha-3 isoform 2
NCBI Official Synonym Full Names
laminin subunit alpha 3
NCBI Official Symbol
LAMA3??[Similar Products]
NCBI Official Synonym Symbols
E170; LOCS; BM600; LAMNA
??[Similar Products]
NCBI Protein Information
laminin subunit alpha-3
UniProt Protein Name
Laminin subunit alpha-3
UniProt Synonym Protein Names
Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
UniProt Gene Name
LAMA3??[Similar Products]
UniProt Synonym Gene Names
LAMNA; E170??[Similar Products]
NCBI Summary for LAMA3
The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Comments for LAMA3
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Research Articles on LAMA3
1. Seventeen percent of the patients were compound heterozygous or homozygous for mutations in the gene LAMA3, 59% carried mutations in both alleles of LAMB3, and 12% were homozygous for mutations in LAMC2. In nine patients with severe generalized JEB, detection of two mutations in one of the genes LAMA3, LAMB3, or LAMC2 was not possible, so the molecular basis of disease could not be clarified completely
Precautions
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Disclaimer
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