Product Name
CD40, Recombinant Protein
Full Product Name
CD40 ligand, 112-260aa, Mouse, His tag, Baculovirus
Product Synonym Names
CD40lg; CD154; CD40-L; Cd40l; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP
Product Gene Name
CD40 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKLH HHHHH
3D Structure
ModBase 3D Structure for P27548
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (lot specific)
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of CD40 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD40 recombinant protein
CD40LG, also known as CD40 ligand, belongs to the tumor necrosis factor family. This protein binds to TNFRSF5. CD40LG mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. CD40 ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. Recombinant mouse CD40LG, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD40 recombinant protein
Immunology
Applications Tested/Suitable for CD40 recombinant protein
SDS-PAGE
SDS-Page of CD40 recombinant protein
NCBI/Uniprot data below describe general gene information for CD40. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035746
[Other Products]
NCBI GenBank Nucleotide #
NM_011616.2
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UniProt Primary Accession #
P27548
[Other Products]
UniProt Related Accession #
P27548[Other Products]
Molecular Weight
17.2kDa (155aa) 18-28KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
Cd40lg??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
??[Similar Products]
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP
Protein Family
CD40 ligand
UniProt Gene Name
Cd40lg??[Similar Products]
UniProt Synonym Gene Names
Cd40l; Tnfsf5; CD40-L; TRAP??[Similar Products]
UniProt Comments for CD40
Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).
Product References and Citations for CD40 recombinant protein
Price JD et al., (2014) J. Leukoc. Biol. 95 (2):325-336.; Williams JA et al., (2014) J. Immunol. 192 (2):630-640.
Research Articles on CD40
1. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in focal segmental glomerulosclerosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
