Product Name
LAMA3, siRNA
Full Product Name
LAMA3 siRNA (Mouse)
Product Synonym Names
Laminin subunit alpha-3; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
Product Gene Name
LAMA3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61789
Specificity
LAMA3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LAMA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LAMA3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LAMA3 sirna
siRNA to inhibit LAMA3 expression using RNA interference
Applications Tested/Suitable for LAMA3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LAMA3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034810.1
[Other Products]
NCBI GenBank Nucleotide #
NM_010680.1
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UniProt Primary Accession #
Q61789
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UniProt Secondary Accession #
O08751; Q61788; Q61966; Q9JHQ7; E9PUR4[Other Products]
UniProt Related Accession #
Q61789[Other Products]
Molecular Weight
190,610 Da
NCBI Official Full Name
laminin subunit alpha-3
NCBI Official Synonym Full Names
laminin, alpha 3
NCBI Official Symbol
Lama3??[Similar Products]
NCBI Official Synonym Symbols
[a]3B; Lama3B
??[Similar Products]
NCBI Protein Information
laminin subunit alpha-3
UniProt Protein Name
Laminin subunit alpha-3
UniProt Synonym Protein Names
Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
UniProt Gene Name
Lama3??[Similar Products]
UniProt Entry Name
LAMA3_MOUSE
UniProt Comments for LAMA3
LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; laminin-5 complex; laminin-3 complex; laminin-1 complex; cell-cell adherens junction; hemidesmosome; extracellular region; basement membrane
Molecular Function: receptor binding
Biological Process: regulation of cell adhesion; cell-cell adhesion; hemidesmosome assembly; regulation of embryonic development; cell adhesion; regulation of cell migration
Research Articles on LAMA3
1. The expression of integrin-alpha6beta4 and laminin-5 (alpha3, beta3, gamma2) mRNAs was significantly higher on SA-treated c.p.Ti than on c.p.Ti and AO c.p.Ti disks after 3 days (P<0.05).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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