Product Name
LAMA3/Laminin 5, Monoclonal Antibody
Full Product Name
Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody
Product Synonym Names
Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; LAMA3; LAMNA
Product Gene Name
anti-LAMA3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16787
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human LAMA3
Subcellular Localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
Tissue Specificity
Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-LAMA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LAMA3 antibody
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Product Categories/Family for anti-LAMA3 antibody
Signal Transduction; Cytoskeleton/ECM; Basal Lamina; Neuroscience; cell adhesion proteins; ECM Proteins; Signal Transduction; Extracellular Matrix; Other ecm proteins
Applications Tested/Suitable for anti-LAMA3 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-LAMA3 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Western Blot (WB) of anti-LAMA3 antibody
Western blot analysis of LAMA3 expression in HeLa cell lysate (MBS1750009).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMA3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LAMA3
NCBI/Uniprot data below describe general gene information for LAMA3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000218.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000227.4
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UniProt Primary Accession #
Q16787
[Other Products]
UniProt Secondary Accession #
Q13679; Q13680; Q6VU67; Q6VU68; Q6VU69; Q76E14; Q96TG0; B0YJ33[Other Products]
UniProt Related Accession #
Q16787[Other Products]
Molecular Weight
366649 MW
NCBI Official Full Name
laminin subunit alpha-3 isoform 2
NCBI Official Synonym Full Names
laminin subunit alpha 3
NCBI Official Symbol
LAMA3??[Similar Products]
NCBI Official Synonym Symbols
E170; LOCS; BM600; LAMNA
??[Similar Products]
NCBI Protein Information
laminin subunit alpha-3
UniProt Protein Name
Laminin subunit alpha-3
UniProt Synonym Protein Names
Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
UniProt Gene Name
LAMA3??[Similar Products]
UniProt Synonym Gene Names
LAMNA; E170??[Similar Products]
NCBI Summary for LAMA3
The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Comments for LAMA3
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Research Articles on LAMA3
1. Seventeen percent of the patients were compound heterozygous or homozygous for mutations in the gene LAMA3, 59% carried mutations in both alleles of LAMB3, and 12% were homozygous for mutations in LAMC2. In nine patients with severe generalized JEB, detection of two mutations in one of the genes LAMA3, LAMB3, or LAMC2 was not possible, so the molecular basis of disease could not be clarified completely
Precautions
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Disclaimer
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