For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000069.6 (28697903..28728361). Location: 3 A3; 3 12.19 cM

No significant cross-reactivity or interference between Rat GLUT2 and analogues was observed.

Store all reagents at 2-8 degree C

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Small volumes of GLUT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS020970 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucose Transporter 2 (GLUT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLUT2. The ELISA analytical biochemical technique of the MBS020970 kit is based on GLUT2 antibody-GLUT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLUT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLUT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GLUT2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GLUT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLUT2 and GLUT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

57,107 Da

solute carrier family 2 (facilitated glucose transporter), member 2

solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver

Solute carrier family 2, facilitated glucose transporter member 2

Glut-2; Glut2; GLUT-2??[Similar Products]

GTR2_MOUSE

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues.

Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Cellular Component: membrane; basolateral plasma membrane; brush border membrane; cytoplasm; integral to membrane; plasma membrane; cytosol; endosome; brush border

Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding

Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport

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