T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

For Research Use Only. Not for use in diagnostic procedures.

E Coli

Greater than 90% as determined by SDS-PAGE. (lot specific)

Liquid containing glycerol

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterile filter available upon request.

Low endotoxin available upon request.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of CD40LG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Cardiovascular

(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)

51.2kD

CD40 ligand

CD40 ligand

CD40 ligand

CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]

CD40L_HUMAN

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: external side of plasma membrane; extracellular space; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell differentiation; B cell proliferation; immunoglobulin secretion; inflammatory response; isotype switching; leukocyte adhesion; negative regulation of apoptosis; platelet activation; positive regulation of interleukin-12 production; regulation of immune response; regulation of immunoglobulin secretion; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency With Hyper-igm, Type 1

Cloning of TRAP, a ligand for CD40 on human T cells.Graf D., Korthaeuer U., Mages H.W., Senger G., Kroczek R.A.Eur. J. Immunol. 22:3191-3194(1992) The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor expression of a soluble form of gp39 with B cell co-stimulatory activity.Hollenbaugh D., Grosmaire L.S., Kullas C.D., Chalupny J.N., Braesch-Andersen S., Noelle R.J., Stamenkovic I., Ledbetter J.A., Aruffo A.EMBO J. 11:4313-4321(1992) The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.Aruffo A., Farrington M., Hollenbaugh D., Li X., Milatovich A., Nonoyama S., Bajorath J., Grosmaire L.S., Stenkamp R., Neubauer M., Roberts R.L., Noelle R.J., Ledbetter J.A., Francke U., Ochs H.D.Cell 72:291-300(1993) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.Spriggs M.K., Armitage R.J., Strockbine L., Clifford K.N., Macduff B.M., Sato T.A., Maliszewski C.R., Fanslow W.C.J. Exp. Med. 176:1543-1550(1992) Human CD40-ligand molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.Gauchat J.-F., Aubry J.-P., Mazzei G.J., Life P., Jomotte T., Elson G., Bonnefoy J.-Y.FEBS Lett. 315:259-266(1993) Structural organization of the gene for CD40 ligand molecular analysis for diagnosis of X-linked hyper-IgM syndrome.Shimadzu M., Nunoi H., Terasaki H., Ninomiya R., Iwata M., Kanegasaka S., Matsuda I.Biochim. Biophys. Acta 1260:67-72(1995) Human native soluble CD40L is a biologically active trimer, processed inside microsomes.Pietravalle F., Lecoanet-Henchoz S., Blasey H., Aubry J.-P., Elson G., Edgerton M.D., Bonnefoy J.-Y., Gauchat J.-F.J. Biol. Chem. 271:5965-5967(1996) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.Khandekar S.S., Silverman C., Wells-Marani J., Bacon A.M., Birrell H., Brigham-Burke M., De;Marini D.J., Jonak Z.L., Camilleri P., Fishman-Lobell J.Protein Expr. Purif. 23:301-310(2001) Release of soluble CD40L from platelets is regulated by glycoprotein IIb/IIIa and actin polymerization.Furman M.I., Krueger L.A., Linden M.D., Barnard M.R., Frelinger A.L. III, Michelson A.D.J. Am. Coll. Cardiol. 43:2319-2325(2004) 2-A crystal structure of an extracellular fragment of human CD40 ligand.Karpsusas M., Hsu Y.-M., Wang J.-H., Thompson J., Lederman S., Chess L., Thomas D.Structure 3:1031-1039(1995) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.Singh J., Garber E., van Vlijmen H., Karpsusas M., Hsu Y.-M., Zheng Z., Naismith J.H., Thomas D.Protein Sci. 7:1124-1135(1998) Defective expression of T-cell CD40 ligand causes X-linked immunodeficiency with hyper-IgM.Korthaeuer U., Graf D., Mages H.W., Briere F., Padayachee M., Malcolm S., Ugazio A.G., Notarangelo L.D., Levinsky R.J., Kroczek R.A.Nature 361:539-541(1993) CD40 ligand mutations in X-linked immunodeficiency with hyper-IgM.Disanto J.P., Bonnefoy J.-Y., Gauchat J.-F., Fischer A., de Saint Basile G.Nature 361:541-543(1993) CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.Allen R.C., Armitage R.J., Conley M.E., Rosenblatt H., Jenkins N.A., Copeland N.G., Bedell M.A., Edelhoff S., Disteche C.M., Simoneaux D.K., Fanslow W.C., Belmont J.W., Spriggs M.K.Science 259:990-993(1993) Characterization of nine novel mutations in the CD40 ligand gene in patients with X-linked hyper IgM syndrome of various ancestry.Macchi P., Villa A., Strina D., Sacco M.G., Morali F., Brugnoni D., Giliani S., Mantuano E., Fasth A., Andersson B., Zegers B.J.M., Cavagni G., Reznick I., Levy J., Zan-Bar I., Porat Y., Airo P., Plebani A., Vezzoni P., Notarangelo L.D.Am. J. Hum. Genet. 56:898-906(1995) Signaling through CD40 rescues IgE but not IgG or IgA secretion in X-linked immunodeficiency with hyper-IgM.Saiki O., Tanaka T., Wada Y., Uda H., Inoue A., Katada Y., Izeki M., Iwata M., Nunoi H., Matsuda I.J. Clin. Invest. 95:510-514(1995) Mutation analysis in CD40 ligand deficiency leading to X-linked hypogammaglobulinemia with hyper IgM syndrome.Katz F., Hinshelwood S., Rutland P., Jones A., Kinnon C., Morgan G.3.0.CO;2-A>Hum. Mutat. 8:223-228(1996) A single strand conformation polymorphism study of CD40 ligand. Efficient mutation analysis and carrier detection for X-linked hyper IgM syndrome.Lin Q., Rohrer J., Allen R.C., Larche M., Greene J.M., Shigeoka A.O., Gatti R.A., Derauf D.C., Belmont J.W., Conley M.E.J. Clin. Invest. 97:196-201(1996) Mutations of the CD40 ligand gene in 13 Japanese patients with X-linked hyper-IgM syndrome.Nonoyama S., Shimadzu M., Toru H., Seyama K., Nunoi H., Neubauer M., Yata J., Och H.D.Hum. Genet. 99:624-627(1997) Mutations of the CD40 ligand gene and its effect on CD40 ligand expression in patients with X-linked hyper IgM syndrome.Seyama K., Nonoyama S., Gangsaas I., Hollenbaugh D., Pabst H.F., Aruffo A., Ochs H.D.Blood 92:2421-2434(1998)

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